Systems and methods for encapsulation and multi-step processing of biological samples

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United States of America Patent

PATENT NO 11860076
APP PUB NO 20200400538A1
SERIAL NO

16934045

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Abstract

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The present invention relates to methods and systems for isolation of species in semi-permeable capsules and processing of encapsulated species through series of steps and/or reactions. To produce capsules, first aqueous two-phase system (ATPS) droplets are generated using microfluidics system and then the hydrogel shell layer is hardened by inducing polymerization. As exemplified in this invention to achieve concentric ATPS droplet formation density-matched PEGDA and Dextran polymer solutions can be used. Once a capsule is formed, its composition can be changed by adding new reagents or replacing out old ones (e.g. by resuspending capsules in desired aqueous solution). The hydrogel shell of semi-permeable capsules can be dissolved at selected step during multi-step procedures in order to release the encapsulated species. The present invention exemplifies the isolation of individual cells within capsules and using the encapsulated cells for genotypic and phenotypic analysis. Finally, the present invention also exemplifies the use of capsules in multi-step procedures to perform complex biological reactions.

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Patent Owner(s)

  • VILNIUS UNIVERSITY; DROPLET GENOMICS, UAB

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Leonavicius, Karolis Vilnius, LT 4 1
Mazutis, Linas Vilnius, LT 19 336
Stonyte, Greta Vilnius, LT 3 5
Zelvyte, Ausra Vilnius, LT 2 1

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