Phosphorescence oxygen analyzer and uses thereof
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United States of America Patent
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Oct 11, 2022
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N/A
app pub date -
Dec 23, 2021
filing date -
Dec 23, 2021
priority date (Note) -
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Abstract
The phosphorescence oxygen analyzer has a light source including an LED array that flashes light at 1,000 flashes per second. The light flashes are received in a test chamber containing a carousel having a plurality (preferably ten) of sample vials mounted thereon. The samples a phosphorescent probe (palladium(II) complex, namely, meso-tetra-(4-sulfonatophenyl)tetrabenzoporphyrin; Pd phosphor) mixed with either a control sample of tissue or a sample of tissue and a suspected toxin or a pharmaceutical it is desired to test, the carousel being rotated to irradiate each vial in turn. The probe has an absorption maximum at 625 nm and emission maximum at 800 nm. Phosphorescent emissions are detected by a photomultiplier tube connected to a measurement 2020 board, which is connected to a processor that computes the lifetime and peak of the pulses, which determines the rate of phosphorescent decay due to oxygen metabolized by the tissue mitochondria.
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