LTD.sub.4 receptor and photoaffinity probe
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United States of America Patent
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May 10, 1994
Grant Date -
N/A
app pub date -
Apr 3, 1992
filing date -
Apr 3, 1992
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The LTD.sub.4 receptor has been defined by radioligand binding studies as a member of the family of G-protein coupled receptors. A photoactivable azido derivative of LTD.sub.4 ([.sup.125 I]Azido-LTD.sub.4) has been synthesized for use as a photoaffinity probe. Photoactivation of ([.sup.125 I]Azido-LTD.sub.4 under equilibrium binding conditions revealed the selective radiolabeling of a 45 kDa protein in guinea-pig lung membranes, as visualized by SDS-PAGE and autoradiography.
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