Method for nucleic acid detection by guiding through a nanopore

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United States of America Patent

PATENT NO 11739379
SERIAL NO

17361821

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Abstract

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The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

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Patent Owner(s)

  • OXFORD NANOPORE TECHNOLOGIES PLC

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bowen, Rebecca Victoria Oxford, GB 24 212
Brown, Clive Gavin Cambridge, GB 68 984
Fordham, Daniel George Oxford, GB 12 47
Graham, James Edward Oxford, GB 14 16
Gutierrez, Richard Alexander Oxford, GB 3 9
Heron, Andrew John Oxford, GB 83 1414
White, James Oxford, GB 193 3135

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