Drosophila clipped FRT (cFRT) chromosome insensitive to P transposase, generating method thereof, and application thereof
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Nov 8, 2005
Grant Date -
Sep 4, 2003
app pub date -
Jan 10, 2002
filing date -
Jan 10, 2002
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
A method for generating a Drosophila clipped FRT (cFRT) chromosome is provided, wherein the chromosome is incapable of reacting with a P transposase but remains capable of reacting with a yeast site-specific flippase recombinase (FLP). The method includes steps of: (a) exposing a FRT chromosome to the P transposase causing a local and imprecise transposition, wherein the FRT chromosome contains a P[FRT] insertion with a selection marker gene, (b) screening the P[FRT] insertion insensitive to the P transposase to obtain screened products, (c) selecting candidate products from the screened products by further examinations, and (d) exposing the candidate products by the P transposase and selecting a desired product by the further examinations to obtain the Drosophila clipped FRT (cFRT) chromosome incapable of reacting with the P transposase but remaining capable of reacting with the yeast site-specific flippase recombinase. The cFRT2L2R chromosome can be used as the direct target in the direct P-transposon-induced mutagenesis.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Advertisement
Advertisement
Advertisement
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully