Method for detecting single strand breaks in DNA

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United States of America Patent

PATENT NO 11898200
APP PUB NO 20210071233A1
SERIAL NO

17014322

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Abstract

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The present disclosure discloses a method for detecting single strand breaks (SSBs) in DNA based on the following steps. First, DNA of interest is fragmented with a method that generates 3′ ends that cannot be tailed. Second, the available 3′ ends of the fragmented DNA corresponding to the pre-existing breaks are tailed. Third, SSBs are captured and their positions are identified genome-wide based on the following steps: (1) the tailed fragments are linearly amplified using a chimeric 5′-DNA-RNA-3′ primer; (2) the products of primer extension are tailed at the 3′ ends; (3) the desired products are amplified by PCR with oligonucleotides containing Illumina® adaptor sequences complementary to both tails and subjected to next-generation sequencing (NGS); 4) finally, positions of SSBs are revealed through the analysis of sequencing results.

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Patent Owner(s)

Patent OwnerAddress
HUAQIAO UNIVERSITYNO 269 CHENGHUA NORTH ROAD QUANZHOU CITY FUJIAN PROVINCE 362000 QUANZHOU CITY FUJIAN PROVINCE 362000

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cai, Ye Fujian, CN 14 2
Cao, Huifen Fujian, CN 1 0
Gao, Fan Fujian, CN 21 19
Han, Xueer Fujian, CN 1 0
Kapranov, Philipp Fujian, CN 9 5
Salazar-García, Lorena Fujian, CN 1 0
Tang, Lu Fujian, CN 5 10
Wang, Fang Fujian, CN 294 2829
Xu, Dongyang Fujian, CN 5 3

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