Methods and reagents for staining intracellular components
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United States of America Patent
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Oct 1, 1991
Issued Date -
N/A
app pub date -
Sep 14, 1989
filing date -
Sep 12, 1988
priority date (Note) -
Expired
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Abstract
Reagents and methods for intracellular staining of anionic materials as provided. The inherent staining characteristics of a fluorescent molecule may be suitably altered by covalently linking a plurality of said molecules to a nonimmunoglobulin derived cationic polymer having the desired staining specificity. By careful selection of the net charge and size of the dye polymer complex, diffusion through the cellular membrane can be enhanced and an enhanced fluorescent signal obtained by localization of a plurality of fluorescent molecules at the site of interest.
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