Method for preparing 1,3-propanediol by a recombinant micro-organism in the absence of coenzyme b12 or one of its precursors
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United States of America Patent
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Sep 11, 2007
Grant Date -
Sep 18, 2003
app pub date -
Jan 9, 2002
filing date -
Jul 9, 1999
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Abstract
The invention concerns a method for preparing 1,3-propanediol from a carbon-containing substance, said method comprising a step which consists in culturing a recombinant micro-organism not producing coenzyme B12 in the absence of coenzyme B12 or one of its precursors. The invention also concerns a nucleic acid coding for a glycerol dehydratase whereof the catalytic activity is independent of the presence of coenzyme B12 or one of its precursors and a nucleic acid coding for a 1,3-propanol dehydrogenase intervening in the synthesis of 1,3-propanediol. The invention further concerns recombinant vectors and host cells comprising said nucleic acids and the polypeptides coded by the latter.
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