Optimizing the initialization and convergence of active contours for segmentation of cell nuclei in histological sections
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United States of America Patent
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Jan 27, 2015
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Mar 11, 2011
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Mar 12, 2010
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Abstract
A computer-implemented segmentation method is used to process an image representing a plurality of nuclei. The method is implemented in a computer having a processor and a physical memory. A set of instructions are provided to the processor the physical memory of the computer. The processor is configured by executing the set of instructions in the physical memory so as to automatically segment the image by: thresholding a grey-scale image to create a black and white image; identifying objects in the black and white image and removing objects failing to meet predetermined criteria; extracting objects; and applying an edge detector on the segmented image to identify the edges of the nuclei. Overlapping nuclei are split to improve results.
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