Microbial production of indigo
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United States of America Patent
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Oct 16, 2001
Issued Date -
N/A
app pub date -
Nov 21, 2000
filing date -
Nov 20, 1995
priority date (Note) -
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Abstract
There is provided an improved process for the biosynthetic production of indigo, the improvement comprising removing unwanted by-products such as isatin or indirubin from the broth in which such indigo is produced. Isatin can be removed by enzymatic activity using an isatin-removing enzyme such as an isatin hydrolase, or by other techniques such as process parameters (elevated temperature, pH), or by contacting the broth containing the isatin with appropriate adsorption compounds/compositions such as carbon or appropriate resins. Since isatin is the precursor of indirubin, the indirubin levels are decreased as a result of isatin removal.
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