Immuno-PCR method for detecting nasopharyngeal carcinoma markers and kit thereof
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United States of America Patent
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Oct 5, 2010
Grant Date -
Jul 23, 2009
app pub date -
Sep 24, 2008
filing date -
Jan 21, 2008
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Abstract
The present invention is related to an immuno-PCR method for detecting nasopharyngeal carcinoma (NPC) and kit thereof, especially related to an immuno-PCR method for detecting markers of early stage NPC and kit thereof. The present invention includes providing a substrate whereon protein markers immobilized; applying a patient's specimen to the substrate; adding a solution which has biotinylated anti-human IgA secondary antibody and incubating the solution; adding a solution with a linker and biotinylated target DNA; proceeding a polymerase chain reaction; and finally, detecting the target DNA fragments via electrophoresis.
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