Analysis for total cholesterol using lecithin:cholesterol acyl transferase (LCAT)
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United States of America Patent
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Feb 12, 1985
Grant Date -
N/A
app pub date -
Jun 1, 1982
filing date -
Jun 1, 1982
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Methods, compositions and elements for the analysis of total cholesterol in aqueous fluids containing cholesterol and cholesterol esters are disclosed. Cholesterol esters in the fluid samples are first converted to cholesterol by: (a) contacting a sample of the fluid with sufficient amounts of lysolecithin and an enzyme exhibiting lecithin:cholesterol acyl transferase (LCAT) activity to convert substantially all the cholesterol esters to cholesterol in the sample, and (b) removing cholesterol from the aqueous fluid other than by conversion to cholesterol esters. The total cholesterol is quantitatively determined by measuring the amount of the removed cholesterol. For example, the amount is measured gravimetrically or by converting the cholesterol to a detectable product.
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