Recombinant nucleic acid containing a response element
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United States of America Patent
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Nov 25, 1997
Grant Date -
N/A
app pub date -
Dec 31, 1992
filing date -
Dec 31, 1992
priority date (Note) -
Expired
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Abstract
The present invention describes a novel method for screening retinoid X receptor agonists or antagonists, comprising: using a retinoid X receptor expressed by a yeast expression system to screen a compound having a retinoid X receptor agonist or antagonist activity as well as a screen for detecting a compound having retinoid X receptor agonist or antagonist activity, which comprises the steps of (1) providing a yeast strain which expresses the retinoic acid receptor and activates a reporter plasmid containing apolipoprotein AI gene site A or a mutated variant thereof; (2) incubating the compound in suitable media and a colorless chromogenic substrate; and (3) examining the media for development of color.
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