Purification of HER-2 variants
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United States of America Patent
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Jul 27, 2010
Grant Date -
Oct 26, 2006
app pub date -
Jun 24, 2004
filing date -
Jun 25, 2003
priority date (Note) -
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Abstract
The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), c) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein for which the purification process has been especially adapted, as well as means for the preparation of the variant.
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