Importance

Loading Importance Indicators...

Abstract

Biochemical assays that are performed in cuvettes and in the wells of multi-well plates and that utilize excitation and light emission as labels for detection are enhanced by an illumination and detection system that supplies excitation light through an optical fiber that transmits excitation light from an excitation light source to the cuvette or well. Emission light produced by the excitation is then collected by a collimating lens and converted to a signal that is compiled by conventional software for analysis. The optical fiber and collimating lens can either be on the same side of the receptacle (generally the open side) or on opposite sides, i.e., one above and the other below. When the optical fiber and the collimating lens are both on the open side of the receptacle, they are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle. Illumination systems are also disclosed in which a ultraviolet, visible, or near-infrared light source is optically coupled to an optical fiber.

Loading the Abstract Image...

First Claim