Sandwich immunoassay utilizing a separation specific binding substance
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United States of America Patent
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Apr 12, 1988
Grant Date -
N/A
app pub date -
Dec 12, 1984
filing date -
Dec 12, 1984
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Immunoassay system for determining the amount of a polyvalent ligand in a liquid test sample, and based on: (a) an insoluble phase to which is bound a separation specific binding substance which does not bind said ligand; (b) a capture specific binding substance which specifically binds said ligand, and which is itself specifically bound by said spearation specific binding substance; and (c) a probe specific binding substance which specifically binds said ligand at a site which is accessible when said ligand is bound to said capture specific binding substance, said probe specific binding substance being not substantially bound by said separation specific binding substance, and being detectable by at lease one detection procedure.
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