Apparatus for sequencing of nucleic acids using multiple dyes
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United States of America Patent
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Aug 27, 2002
Issued Date -
N/A
app pub date -
Dec 9, 1998
filing date -
Apr 18, 1996
priority date (Note) -
Expired
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Abstract
An instrument for sequencing oligonucleotides is loaded with the products of four sequencing reaction mixtures. These products are a combination of A, C, G and T reaction products for several sequencing reactions. The products of the different sequencing reactions are labeled with fluorescent tags which are distinguishable one from the other on the basis of their excitation or emission spectra. After separation of the oligonucleotides by electrophoresis, the order of the detected peaks is used to call the base sequence.
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