Methods and compositions for tracking nucleic acid fragment origin for nucleic acid sequencing

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United States of America Patent

PATENT NO 11807903
APP PUB NO 20210047683A1
SERIAL NO

16968576

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Abstract

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The present disclosure provides methods and compositions for tracking nucleic acid fragment origin by target-specific barcode tagging when original nucleic acid targets break into small fragments. Nucleic acid targets are captured in vitro on a solid support with clonally localized nucleic acid barcode templates. Many nucleic acid targets canbe processed simultaneously in a massively parallel fashion without partition. These nucleic acid target tracking methods can be used for a variety of applications in both whole genome sequencing and targeted sequencing in order to accurately identify genomic variants, haplotype phasing and assembly, for example.

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Patent Owner(s)

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UNIVERSAL SEQUENCING TECHNOLOGY CORPORATION780 DEDHAM STREET CANTON MA 02021

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Zhoutao Carlsbad, US 25 710
Pham, Long Kim San Diego, US 5 43
Wang, Yong Sharon, US 995 8736
Wu, Tsai-Chin San Marcos, US 4 1

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