Method for synthetic genes
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United States of America
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Mar 8, 2022
Grant Date -
Apr 4, 2019
app pub date -
Dec 11, 2018
filing date -
Dec 11, 2018
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Abstract
The present invention provides a method of designing an optimized gene which comprises altering a nucleotide sequence of a target protein gene, so that only preferential codons with high frequency of use in human cells are selected and a GC content of not less than 60% is achieved. A gene design method which involves the feature “only preferential codons with high frequency of use are selected and a GC content of not less than 60% is achieved” can be established as a general rule for preparing proteins with high expression level, in order to obtain chemically synthesized genes for proteins capable of high-level expression in eukaryotes.
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