Nucleic acid amplification procedure using RNA and DNA composite primers
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United States of America Patent
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May 10, 2011
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May 21, 2009
app pub date -
Sep 7, 2006
filing date -
Sep 7, 2005
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Abstract
The invention provides methods for amplification of polynucleotide sequences using primers containing single-stranded RNA. The methods employ use of an enzyme capable of cleaving single-stranded RNA, such as RNase I, to degrade a first RNA-containing primer prior to addition of a second RNA-containing primer. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
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