Single cell cellular component enrichment from barcoded sequencing libraries

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United States of America Patent

PATENT NO 11702649
APP PUB NO 20200392479A1
SERIAL NO

16758640

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Abstract

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The present invention relates to the analysis of complex single cell sequencing libraries. Disclosed are methods for enrichment of library members based on the presence of cell-of origin barcodes to identify and concentrate DNA that is relevant to interesting cells or components that would be expensive or difficult to study otherwise. Also, disclosed are methods of capturing cDNA library molecules by use of CRISPR systems, hybridization or PCR. The present invention allows for identifying the properties of rare cells in single cell RNA-seq data and accurately profile them through clustering approaches. Further information on transcript abundances from subpopulations of single cells can be analyzed at a lower sequencing effort. The methods also allow for linking TCR alpha and beta chains at the single cell level.

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Patent Owner(s)

  • THE BROAD INSTITUTE, INC.; MASSACHUSETTS INSTITUTE OF TECHNOLOGY

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Blainey, Paul Cambridge, US 46 323
Gierahn, Todd Cambridge, US 28 182
Love, J Christopher Cambridge, US 42 437
Ranu, Navpreet Cambridge, US 4 0

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