Stably maintained multiple copies of at least two ORF in the same orientation

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United States of America Patent

PATENT NO 10640757
APP PUB NO 20140106457A1
SERIAL NO

14034124

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Abstract

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The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of:

    a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position;b) integrating the at least two ORF's in the same orientation on the host cell chromosome.

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Patent Owner(s)

  • NOVOZYMES A/S

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Jorgensen, Steen Troels Allerod, DK 18 132
Pedersen, Poul Erik Soborg, DK 32 118

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