Donor plasmid vectors

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United States of America Patent

PATENT NO 11674157
APP PUB NO 20190002918A1
SERIAL NO

16012686

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Abstract

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Certain donor plasmid vectors such as pFastBac™1 and pFastBac™ Dual lack a cis DNA element upstream of the polh translation start codon (ATG) present in wild type (wt) Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and contain a SV40 pA fragment. When a cis DNA element is inserted upstream of the 50 bp polh promoter and SV40 pA was replaced with a AcMNPV polh pA signal in pFastBac™1 and pFastBac™Dual, certain protein expression levels in High Five™ cells using the Bac-to-Bac® system reached that of the wt AcMNPV.

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Patent Owner(s)

  • MIAMI UNIVERSITY

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cheng, Xiao-Wen Oxford, US 3 5
Garretson, Tyler Oxford, US 1 0
Shang, Hui Oxford, US 1 0

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