Nucleic acid amplification using a reversibly inactivated thermostable enzyme
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United States of America Patent
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Jun 30, 1998
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Jul 11, 1996
filing date -
Aug 25, 1995
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Abstract
The present invention provides methods for the amplification of nucleic acids using a reversibly inactivated thermostable enzyme. The reversibly inactivated enzyme is the result of a chemical modification of the protein which inactivates the enzyme. The activity of the inactivated enzyme is recovered by an incubation of the reaction mixture at an elevated temperature prior to, or as part of, the amplification reaction. Non-specific amplification is reduced because the reaction mixture does not support the formation of extension products prior to the activating incubation.
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