Recombinant mycobacteria overexpressing D-alanine ligase gene and uses therefore
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United States of America Patent
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May 13, 2008
Grant Date -
Dec 2, 2004
app pub date -
Dec 17, 2003
filing date -
Dec 17, 2002
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Abstract
Recombinant mycobacterial strains which overproduce essential biosynthetic enzymes of pathogenic mycobateria are provided. These strains overproduce enzymes involved in the synthesis and incorporation of D-alanine into mycobacterial peptidoglycan, the backbone of the mycobacterial cell wall. These overproducing strains may be used as reference strains in in vitro screening methods to identify antimycobacterial agents.
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