Method and system for determining integrated metabolic baseline and potential of living cells

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 11940442
APP PUB NO 20200333327A1
SERIAL NO

16917352

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The current technology is related to methods for rapidly determining the metabolic baseline and potential of living cells. Embodiments relate to measuring the activity of each of the two major energy-generating pathways within the cell: mitochondrial respiration and glycolysis, first under baseline conditions, and again after applying a stress to the cells to demand increased energy supply. In some embodiments the stress may be applied by exposing the cells to a combination of two chemical compounds: a mitochondrial uncoupler and an ATP synthase inhibitor. In one embodiment, the metabolic energy generating activity of the mitochondrial respiration pathway is determined by measuring the rate of oxygen consumption by the living cells, and the metabolic energy generating activity of the glycolysis pathway is determined from a measurement of extracellular acidification caused by secretion of protons from the cell. Other embodiments are related to an apparatus for determining a metabolic potential of a cell sample in a well of a multiwell plate.

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Patent Owner(s)

  • AGILENT TECHNOLOGIES, INC.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Dranka, Brian Acton, US 2 0
Ferrick, David A El Macero, US 9 39

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