Method for detecting on-target and predicted off-target genome editing events

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United States of America Patent

PATENT NO 11926866
APP PUB NO 20210202034A1
SERIAL NO

17140640

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Abstract

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Methods for detecting on-target and predicted off-target genome editing events by providing a multiplex PCR reaction mixture with an on-target oligonucleotide primer and one or more off-target oligonucleotide primers and then hybridizing the on-target oligonucleotide primer and the one or more off-target oligonucleotide primers to target nucleic acid sequences, followed by cleaving blocking groups from the primers and extending the primers.

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Patent Owner(s)

  • INTEGRATED DNA TECHNOLOGIES, INC.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Behlke, Mark Aaron Coralville, US 68 828
Chen, Caifu Palo Alto, US 73 1169
Dobosy, Joseph Coralville, US 21 212
Rettig, Garrett Richard Coralville, US 13 74

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