Method to assay coenzyme Q.sub.10 in blood plasma or blood serum
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United States of America Patent
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Dec 4, 2007
Grant Date -
Feb 19, 2004
app pub date -
May 23, 2003
filing date -
May 23, 2002
priority date (Note) -
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Abstract
A method is described for determining CoQ.sub.10 concentrations in plasma samples. CoQ.sub.10 in the plasma sample is oxidized by treating the sample with an oxidizing agent having a redox potential higher than the redox potential of CoQ.sub.10, such as, for example, para-benzoquinone. Following oxidation of the CoQ.sub.10, the CoQ.sub.10 in the plasma sample is extracted with an alcohol, such as, for example, 1-propanol. The alcohol extract is analyzed using direct injection into the HPLC apparatus. This method achieves a rapid, accurate analysis of plasma CoQ.sub.10 levels, which can be used for monitoring the bioavailability of orally administered CoQ.sub.10 used as a food supplement or as an adjunctive therapy.
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