Method of regulating gene expression in a cell

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United States of America Patent

PATENT NO 11384358
APP PUB NO 20200109407A1
SERIAL NO

16498465

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Abstract

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The disclosure provides methods of making a tetracycline inducible expression system in a cell. The methods include providing the cell with a first nucleic acid sequence comprising a first promoter operably linked to a tetracycline repressor gene coding sequence, providing the cell with a second nucleic acid sequence comprising a second promoter operably linked to a coding sequence of a gene of interest wherein the second promoter is modified to include one or more tetracycline repressor protein binding sites, and determining the expression of the gene of interest in the presence or absence of tetracycline. The disclosure further provides nucleic acid sequences, vectors and cells including the tetracycline inducible modified promoter.

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Patent Owner(s)

Patent OwnerAddress
PRESIDENT AND FELLOWS OF HARVARD COLLEGE17 QUINCY STREET CAMBRIDGE MA 02138

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Church, George M Brookline, US 272 10665
Debnath, Anik Boston, US 5 0
Juarez, Javier Fernandez Cambridge, US 3 9
Lee, Henry Hung-yi Brookline, US 7 3

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