System for qualitatively and/or quantitatively analyzing preferably biological substances using enhanced chemiluminescence, and method and analysis kit using same
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United States of America Patent
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Sep 26, 2000
Grant Date -
N/A
app pub date -
May 9, 1998
filing date -
Oct 20, 1995
priority date (Note) -
Expired
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Abstract
The field of the present invention is that of identification and analysis of chemical and/or biological species of the enzyme/substrate, enzyme/inhibitor or antigen/antibody etc. type. The problem on which the invention is based is to provide a system for qualitative and/or quantitative analysis of biological substances by amplified chemiluminescence which allows an actual significant improvement in the emission of light resulting from passage of a chemiluminescent reagent to the excited state. This problem has been solved by means of a system according to the invention, which involves a ligand a) which can be coupled with the substances to be analysed, a chemiluminescent reagent b) of the luminol type, an enzyme c), a substrate d) which oxidizes the enzyme c), and at least one amplifier e), this system being characterized in that the amplifier e) is chosen from the family of halogenophenol (iodophenol) esters. The invention also relates to an analytical method using this system and to an analytical kit comprising the said system.
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