Diagnostic applications of double D-loop formation
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United States of America Patent
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Sep 23, 1997
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N/A
app pub date -
May 25, 1994
filing date -
May 7, 1990
priority date (Note) -
Expired
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Abstract
The present invention describes the formation of RecA protein catalyzed double-stranded probe:duplex linear target DNA complexes that are stable to deproteinization. The uses of this stable probe:target complex in diagnostic/DNA detection systems in in vitro and in situ DNA hybridization reactions is discussed. The probe:target complexes are also useful for diagnostic application in RecA protein facilitated DNA amplification reactions.
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