Method for producing intestinal organoid derived from pluripotent stem cells

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United States of America Patent

PATENT NO 11859212
APP PUB NO 20200157507A1
SERIAL NO

16611359

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Abstract

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An object of the present invention is to prepare a functional intestinal organoid from pluripotent stem cells. An intestinal organoid is prepared from pluripotent stem cells, by the following steps (1) to (4): (1) differentiating pluripotent stem cells into endoderm-like cells; (2) differentiating the endoderm-like cells obtained in step (1) into intestinal stem cell-like cells; (3) culturing the intestinal stem cell-like cells obtained in step (2) to form spheroids; and (4) differentiating the spheroids formed in step (3) to form an intestinal organoid, the step including culture in the presence of a MEK1/2 inhibitor, a DNA methylation inhibitor, a TGF-β receptor inhibitor, and a γ-secretase inhibitor, in addition to an epidermal growth factor, a BMP inhibitor, and a Wnt signal activator.

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Patent Owner(s)

Patent OwnerAddress
PUBLIC UNIVERSITY CORPORATION NAGOYA CITY UNIVERSITY1 AZA KAWASUMI MIZUHO-CHO MIZUHO-KU NAGOYA-CITY AICHI 467-8601

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Iwao, Takahiro Nagoya, JP 6 2
Matsunaga, Tamihide Nagoya, JP 8 2
Ogawa, Isamu Nagoya, JP 14 212
Onozato, Daichi Nagoya, JP 3 1

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