Altered recombinases for genome modification
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United States of America Patent
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Jun 8, 2010
Grant Date -
Apr 5, 2007
app pub date -
Oct 17, 2006
filing date -
Feb 18, 2000
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Abstract
The present invention describes methods of identifying altered recombinases and compositions thereof, wherein at least one amino acid is different from a parent, wild-type recombinase and the altered recombinase has improved recombination efficiency towards wild-type and/or pseudo att site sequences relative to the parent, wild-type recombinase. The present invention also includes methods of modifying the genomes of cells using the altered recombinases, including methods of site-specifically integrating a polynucleotide sequence of interest in a genome of a eucaryotic cell.
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