Amine detection method and materials
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United States of America Patent
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-
Jun 12, 2007
Grant Date -
Jun 5, 2003
app pub date -
Oct 25, 2001
filing date -
Oct 25, 2001
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A compound linked to a solid support (R) through a divalent linker moiety (X) and which is represented by the following formula: ##STR00001## is disclosed. In particular, the 1-hydroxybenzotriazole-6-carboxylic acid is directly linked to the support under mild conditions (i.e., in aqueous or organic solvents at neutral pH and at room temperature). The polymer bound 1-hydroxybenzotriazole-6-carboxylic acid can be used for the derivatization of amines as well as for single step amino group modification of proteins, peptides, and amines via acylation or sulfonylation reactions. A flow through device and method for the single step amino group modifications of proteins, peptides, and amines is disclosed. Also disclosed is a flow through device for the detection of amines in a sample. Additionally, a device and method for the detection of amines in a sample using 1-hydroxybenzotriazole-6-carboxylic acid is disclosed. In a preferred embodiment, the device is used to detect the presence of amines in a spoiled meat product. Diagnostic kits for detecting the presence of amines is also disclosed.
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