Importance

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Abstract

Methods are disclosed for preparing (1) a pure, biologically active, immunogenic tumor-associated antigen by forming a soluble pool of membrane proteins from antigenic tissue and, by separating, identifying and characterizing antigenic and/or immunogenic proteins therefrom; (2) an ultrapure, biologically active TAA by further subjecting the pure TAA to isotachophoresis and/or affinity chromatography; and (3) making an epitope from purified TAA by injecting TAA into a host animal to stimulate the production by lymphocytes of antibodies specific to TAA, forming hybridomas capable of secreting monoclonal antibodies reactive with the TAA, dividing the TAA into fragments including epitopes, forming epitope-monoclonal antibody complexes, separating the complexes to recover the epitopes, and thereafter identifying and characterizing the epitopes.

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First Claim