Method of detecting mismatching regions
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United States of America Patent
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Jul 31, 2007
Grant Date -
Jun 16, 2005
app pub date -
Sep 30, 2004
filing date -
Sep 30, 2003
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A labeled polynucleotide is subjected to hybridization with oligonucleotide probes fixed respectively to regions on a supporting material. With a first restriction enzyme, a single-stranded moiety of the labeled polynucleotide having failed to form a double strand with each probe is separated from a double strand having been formed by the labeled polynucleotide and each probe. First detection data is obtained from each region. A mismatching moiety, at which the labeled polynucleotide and a certain probe have undergone a mismatch binding, is separated with a second restriction enzyme, and second detection data is obtained from each region. The region to which the mismatch polynucleotide was bound is specified in accordance with results of a comparison between the first detection data and the second detection data.
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