Genetically engineered strain with high yield of L-valine and method for producing L-valine by fermentation

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 11802300
APP PUB NO 20230107828A1
SERIAL NO

17761215

Stats

ATTORNEY / AGENT: (SPONSORED)

Importance

Loading Importance Indicators... loading....

Abstract

See full text

A genetically engineered strain having high-yield of L-valine is disclosed. Starting from Escherichia coli W3110, an acetolactate synthase gene alsS of Bacillus subtilis is inserted into a genome thereof and overexpressed; a ppGpp 3′-pyrophosphate hydrolase mutant R290E/K292D gene spoTM of Escherichia coli is inserted into the genome and overexpressed; a lactate dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB, and genes frdA, frdB, frdC, frdD of four subunits of fumaric acid reductase are deleted from the genome; a leucine dehydrogenase gene bcd of Bacillus subtilis replaces a branched chain amino acid transaminase gene ilvE of Escherichia coli; and an acetohydroxy acid isomeroreductase mutant L67E/R68F/K75E gene ilvCM replaces the native acetohydroxy acid isomeroreductase gene ilvC of Escherichia coli. Furthermore, the L-valine fermentation method is improved by using a two-stage dissolved oxygen control. The L-valine titer and the sugar-acid conversion rate are increased.

Loading the Abstract Image... loading....

First Claim

See full text

Family

Loading Family data... loading....

Patent Owner(s)

  • TIANJIN UNIVERSITY OF SCIENCE & TECHNOLOGY

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hao, Yanan Tianjin, CN 2 0
Liu, Xiaoqian Tianjin, CN 10 37
Wang, Jiachu Tianjin, CN 1 0
Wu, Faqing Tianjin, CN 1 0
Wu, Heyun Tianjin, CN 4 1
Xie, Xixian Tianjin, CN 7 1

Cited Art Landscape

Load Citation

Patent Citation Ranking

Forward Cite Landscape

Load Citation

Maintenance Fees

Fee Large entity fee small entity fee micro entity fee due date
3.5 Year Payment $1600.00 $800.00 $400.00 Apr 30, 2027
7.5 Year Payment $3600.00 $1800.00 $900.00 Apr 30, 2031
11.5 Year Payment $7400.00 $3700.00 $1850.00 Apr 30, 2035
Fee Large entity fee small entity fee micro entity fee
Surcharge - 3.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge - 7.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge - 11.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge after expiration - Late payment is unavoidable $700.00 $350.00 $175.00
Surcharge after expiration - Late payment is unintentional $1,640.00 $820.00 $410.00