Process for obtaining maltose phosphorylase and .beta.-phosphoglucomutase and method of determining .alpha.-amylase using same

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United States of America Patent

PATENT NO 4237221
SERIAL NO

05953723

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The present invention provides a process for obtaining maltose phosphorylase and/or .beta.-phosphoglucomutase from micro-organisms, wherein the starting material used is selected from Lactobacillus brevis DSM 20054, NCIB 8836, 8561 and 8562, Lactobacillus plantarum DSM 20174 and 43, Lactobacillus reuteri DSM 20016, Lactobacillus fermentum DSM 20052, Streptococcus spec. DSM 1118, DSM 119, DSM 1120 and DSM 1121. The present invention also provides a composition and process for determining .alpha.-amylase, wherein maltose phosphorylase and .beta.-phosphoglucomutase obtained by such a process is used, as a crude extract or in an enriched form, optionally with the addition of .alpha.-glucose-1,6-diphosphate and of divalent manganese ions.

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Patent Owner(s)

  • BOEHRINGER MANNHEIM GMBH

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Gauhl, Helmgard Tutzing, DE 11 81
Seidel, Hans Tutzing, DE 20 121

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