Compositions and methods for detecting phosphomonoester
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United States of America Patent
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Sep 28, 2010
Grant Date -
Jun 14, 2007
app pub date -
Dec 9, 2005
filing date -
Dec 9, 2005
priority date (Note) -
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Abstract
The invention provides a method of modifying a phosphomonoester moiety of a target compound. The method can include the steps of (a) providing a target compound having an electrophilic moiety and a phosphomonoester moiety; (b) contacting the target compound with a first carbodiimide compound under conditions for preferential addition of the first carbodiimide compound to the electrophilic moiety over the phosphomonoester moiety, thereby forming an electrophile-protected target compound; and (c) contacting the electrophile-protected target compound with a second carbodiimide compound and a nucleophilic compound under conditions for addition of the nucleophilic compound to the phosphomonoester.
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