Microarray based multiplex pathogen analysis and uses thereof

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United States of America Patent

PATENT NO 11542498
APP PUB NO 20180251758A1
SERIAL NO

15916062

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Abstract

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Provided herein is a 3-dimensional lattice microarray system for DNA sequence detection and analysis. The system has a plurality of bifunctional polymer linkers, on one end of which are attached nucleic acid probes where each have a sequence complementary to signature nucleotide sequences in pathogens, plants or animals. The other end of the bifunctional polymer linker is attached to a solid support by non-covalent or covalent means. Each of the nucleic acid probes have terminal thymidine bases at the 5′ and 3′ ends that permit attachment of the probes to the bifunctional polymer linkers. Also provided is a method for fabricating the microarray system by first attaching the bifunctional polymer linkers to the solid support, followed by photochemical coupling of the nucleic probes to the microarray. A customizable microarray kit is provided that contains the solid support, linkers, probes, solvent mixture and instructions to use the kit.

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Patent Owner(s)

  • PATHOGENDX INC

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Eggers, Frederick Henry Sahuarita, US 14 36
Hogan, Michael Edward Stony Brook, US 16 36
May, Melissa Rose Tucson, US 10 28

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