Alignment mechanism for two-electrode voltage-clamp perfusion chamber for electrophysiological testing of oocytes
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United States of America Patent
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Oct 8, 2002
Grant Date -
Jul 25, 2002
app pub date -
Jan 25, 2001
filing date -
Jan 25, 2001
priority date (Note) -
Expired
status (Latency Note)
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Abstract
An automated mechanism for guiding a microelectrode toward an oocyte placed in a perfusion chamber consists of a movable guide tube holding the electrode and a fixed guide collar in fixed relation to the target oocyte. Because the guide tube and guide collar are independently mounted, the alignment of the tip of the microelectrode is effected by its placement within the precisely aligned guide collar, irrespective of any fine misalignment of the guide tube. Accordingly, a change of microelectrode in the guide tube does not affect its final alignment toward the oocyte so long as the calibration of the guide collar is not disturbed, thereby providing a mechanism for maintaining the alignment of different microelectrodes successively mounted in the system without requiring recalibration.
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