Sample-collecting and assay device for use in the detection of biological material
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United States of America Patent
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Mar 28, 2000
Grant Date -
N/A
app pub date -
Jan 23, 1998
filing date -
Apr 23, 1993
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
An assay device comprises a tube, a removable top part and a bottom part, wherein an elongate member with a swab at its distal end is mounted on the top part, the top part includes a compartment containing liquid and partly defined by a first frangible membrane that can be ruptured to release the liquid into the tube, and the bottom part contains a reagent and is partly defined by a second frangible membrane that can be ruptured on movement of the bottom part relative to the tube. Reaction occurring in the bottom part can be observed through a window. This reaction may be of the type involving amplification of say, ATP, e.g. by providing AMP, glucose-6-phosphate (G6P), adenylate kinase, glucokinase and reagents that convert glucose (G) to give a color, whereby the following reactions occur AMP+ATP.fwdarw.2ADP G6P+ADP.fwdarw.ATP+G.
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