GENOME EDITING OF ROSA LOCUS USING ZINC FINGER NUCLEASE

Japan Patent

APP PUB NO JP-2016138104-A
SERIAL NO

2016020571

Stats

Importance

Loading Importance Indicators... loading....

Abstract

See full text

PROBLEM TO BE SOLVED: To provide a fusion protein mainly comprised of a nuclease capable of genome-editing of a ROSA gene region known to be ubiquitously expressed.SOLUTION: Provided is a fusion protein in which a nuclease such as a restriction enzyme FokI is fused to a zinc finger designed to have a specific amino acid sequence capable of recognizing the DNA sequence of ROSA gene region. In certain embodiments of the fusion protein, the nuclease comprises a cleavage domain or cleavage half-domain, which is a type IIS restriction endonuclease cleavage domain or a cleavage half-domain.SELECTED DRAWING: Figure 1

Loading the Abstract Image... loading....

First Claim

See full text

Family

Loading Family data... loading....

Patent Owner(s)

Patent OwnerAddress
SANAGAMO BIOSCIENCES INCNot Provided

International Classification(s)

Inventor(s)

Inventor Name Address
CUI XIAOXIA -
PHILIP D GREGORY -
MICHAEL C HOLMES -
GREGORY DAVIS -
EDWARD J WEINSTEIN -

Cited Art Landscape

Load Citation