Nested oligonucleotides containing a hairpin for nucleic acid amplification
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United States of America Patent
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Jun 1, 2010
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N/A
app pub date -
Feb 18, 2009
filing date -
Dec 11, 2000
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Abstract
Templates that are engineered to contain a predetermined sequence and a hairpin structure are provided by a nested oligonucleotide extension reaction. The engineered template allows Single Primer Amplification (SPA) to amplify a target sequence within the engineered template. In particularly useful embodiments, the target sequences from the engineered templates are cloned into expression vehicles to provide a library of polypeptides or proteins, such as, for example, an antibody library.
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