Mouse cell line authentication

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United States of America Patent

PATENT NO RE49835
SERIAL NO

16263835

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Abstract

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A multiplex polymerase chain reaction assay that targets nine tetranucleotide short tandem repeat (STR) markers in the mouse genome. Unique profiles were obtained from seventy-two mouse samples that were used to determine the allele distribution for each STR marker. Correlations between allele fragment length and repeat number were determined with DNA Sanger sequencing. Genotypes for L929 and NIH3T3 cell lines were shown to be stable with increasing passage numbers as there were no significant differences in fragment length with samples of low passage when compared to high passage samples. In order to detect cell line contaminants, primers for two human STR markers were incorporated into the multiplex assay to facilitate detection of human and African green monkey DNA. This multiplex assay is the first of its kind to provide a unique STR profile for each individual mouse sample and can be used to authenticate mouse cell lines.

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Patent Owner(s)

Patent OwnerAddress
GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF COMMERCENATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY 100 BUREAU DRIVE MS 2200 GAITHERSBURG MD 20899

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Almeida, Jamie L Gaithersburg, US 2 0
Cole, Kenneth D Gaithersburg, US 6 56

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