Methods and product for optimising localised or spatial detection of gene expression in a tissue sample

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United States of America

PATENT NO RE50065
SERIAL NO

16353937

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Abstract

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The present invention relates to methods and products for localized or spatial detection and/or analysis of RNA in a tissue sample or a portion thereof, comprising: (a) providing an object substrate on which at least one species of capture probe, comprising a capture domain, is directly or indirectly immobilized such that the probes are oriented to have a free 3′ end to enable said probe to function as a reverse transcriptase (RT) primer; (b) contacting said substrate with a tissue sample and allowing RNA of the tissue sample to hybridise to the capture probes; (c) generating cDNA molecules from the captured RNA molecules using said capture probes as RT primers; (d) labelling the cDNA molecules generated in step (c), wherein said labelling step may be contemporaneous with, or subsequent to, said generating step; (e) detecting a signal from the labelled cDNA molecules; and optionally (f) imaging the tissue sample, wherein the tissue sample is imaged before or after step (c).

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Patent Owner(s)

Patent OwnerAddress
10X GENOMICS SWEDEN AB113 63 STOCKHOLM

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Frisen, Jonas Stockholm, SE 49 3304
Lundeberg, Joakim Lidingo, SE 38 3389
Salmen, Fredrik Stockholm, SE 3 346
Stahl, Patrik Stockholm, SE 28 2309

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