Method of nucleic acid fragmentation

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United States of America Patent

PATENT NO 10450557
APP PUB NO 20150315571A1
SERIAL NO

14716212

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Abstract

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A method of preparing an at least partially randomly sheared library of nucleic acids is provided. The method includes the steps of providing a source of nucleic acids, randomly incorporating modified bases into the nucleic acids, and digesting the modified nucleic acids with one or more modification-dependent restriction endonucleases to produce the nucleic acid library. The practice of the method can be facilitated using a kit for performing the method. The method can be used to form nucleic acid libraries and as part of a method of next generation sequencing.

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Patent Owner(s)

Patent OwnerAddress
AGRICULTURE VICTORIA SERVICES PTY LTDBUNDOORA VIC 3083

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cogan, Noel Oliver Ian Macleod, AU 1 1
Shinozuka, Hiroshi Kingsbury, AU 19 83

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