METHOD FOR EXPANDING PROPAGATION OF METARHIZIUM RILEYI MR006 AND USE THEREOF
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United States of America
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Issued Date -
Dec 12, 2024
app pub date -
Jun 5, 2024
filing date -
Jun 5, 2024
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Abstract
The present invention relates to the field of microbial culture, in particular to a method for expanding propagation and producing spores of Metarhizium rileyi and use thereof. In the present invention, Metarhizium rileyi Mr006 is sprayed on 2nd instar live larvae of Spodoptera litura treated with a sub-lethal dose of chlorantraniliprole to cause infection, the 2nd instar larvae of the Spodoptera litura infected with the Metarhizium rileyi Mr006 spores are concentrated into a sterile box, placed into an artificial climate box and continuously fed with an artificial feed until death or pupation, the infection condition of the insect bodies is observed, spores are collected after a 1st complete spore production, after the infected insects are subjected to moisture preservation, expanded propagation and culture are continuously performed, and the spores are repeatedly collected twice to obtain the maximum spore yield.
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