Novel expression plasmids containing the full cDNA sequence of calf prochymosin

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United States of America Patent

PATENT NO 4757020
SERIAL NO

06706979

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Abstract

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Expression plasmids containing the full cDNA sequence of calf prochymosin and capable of expressing prochymosin gene in E. coli host cells are disclosed. A method is described for the preparation of said plasmids which comprises altering the spacing between the SD and ATG sequences within the E. coli trp promoter-operator region of the parent expression plasmid, pCR 701, which is known to express prochymosin gene in the E. coli host cells. These modified expression plasmids are designed to provide high expression levels of prochymosin.

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Patent Owner(s)

  • TERUHIKO BEPPU

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Beppu, Teruhiko 5-21, Horinouchi-1-chome, Suginami-ku, Tokyo, JP 15 173
Kawaguchi, Yoshiyuki Tokyo, JP 12 109
Nishimori, Katsuhiko Tokyo, JP 2 11
Shimizu, Norio Hitachi, JP 96 935
Uozumi, Takeshi Tokyo, JP 2 6
Yanagida, Noboru Yokohama, JP 18 121

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