OBLIQUE-ILLUMINATION SYSTEMS AND METHODS

World Intellectual Property Organization Patent

APP PUB NO WO-2012037182-A1
SERIAL NO

PCTUS2011051486

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Abstract

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Oblique-illumination systems integrated with fluorescence microscopes and methods of using oblique illumination in fluorescence microscopy are disclosed. An oblique-illumination system is attached to a fluorescence microscope objective. The oblique-illumination system can be used to illuminate from any desired direction the surface of an object located at a fixed known offset away from a sample solution containing fluorescently tagged targets. Oblique illumination is used to illuminate features of the surface while epi-illumination is used to create fluorescent light emitted from the tagged targets. The combination of oblique illumination of the surface and epi-illumination of the targets enables capture of images of the surface features and the fluorescent targets so that the locations of the targets in the sample can be determined based on the locations of the surface features.

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Patent Owner(s)

Patent OwnerAddress
APPLIED PRECISION INCUSISSAQUAH WA 98027

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Inventor(s)

Inventor Name Address
TEPLITZ KYLA SEATTLE WA
BROWN CARL BELLEVUE WA WASHINGTON 98006-1350

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